Open Access Highly Accessed Methodology article

Single nucleotide polymorphism discovery in cutthroat trout subspecies using genome reduction, barcoding, and 454 pyro-sequencing

Derek D Houston1*, David B Elzinga2, Peter J Maughan2, Scott M Smith2, John SK Kauwe1, R Paul Evans3, Ryan B Stinger1 and Dennis K Shiozawa14

Author Affiliations

1 Department of Biology, Brigham Young University, Provo, UT, 84602, USA

2 Department of Plant and Wildlife Sciences, Brigham Young University, Provo, UT, 84602, USA

3 Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, USA

4 Curator of Fishes, Monte L. Bean Life Science Museum, Brigham Young University, Provo, UT, 84602, USA

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BMC Genomics 2012, 13:724  doi:10.1186/1471-2164-13-724

Published: 23 December 2012

Additional files

Additional file 1:

SNP primer table, SNP marker names, GenBank accession numbers, the type of polymorphism for each SNP, allele specific primers, common reverse primers and specific target amplification primers are listed herein.

Format: DOCX Size: 47KB Download file

Open Data

Additional file 2:

Characterization of each SNP locus, Major and minor alleles for all 125 SNP loci, along with the proportions of individuals within each a priori designated subspecies that carry the minor allele, as well as minor allele frequencies for each SNP locus are listed herein.

Format: DOCX Size: 38KB Download file

Open Data