Table 1

Growth of Thermacetogenium phaeum with different substrates in pure and syntrophic co-culture
Pure culture ΔOD600 Cell density [mg · l-1] Substrate utilized [mM] Acetate formed [mM] electron balance [%] Molar growth yield YE[g · mol-1]
Acetoin 0.05 12.5 7.3 10.5 97.5 1.71
2,3 Butanediol 0.046 11.5 4.5 8.3 151.3 2.56
Methanol 0.583 145.8 12.2 7 86.5 11.95
Ethanol 0.047 11.75 15.3 17.2 75.5 0.77
Syntrophic culture
Acetoin 0.377 94.25 7.4 12.5 128.5 12.74
2,3 Butanediol 0.045 11.25 7.7 11.4 120.1 1.46
Methanol 0.02 5 0.2 0.2 - -
Ethanol 0.07 17.5 20.2 15.7 52.3 0.87

Molar growth yields (Y) and electron balance were calculated using the formula <C4H7O3> for cell material and the experimentally determined conversion factor OD600 = 1 = 0.250 g cell mass· L-1.

Data show growth after 4 weeks. In most cases no further growth could be observed.

Oehler et al.

Oehler et al. BMC Genomics 2012 13:723   doi:10.1186/1471-2164-13-723

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