Table 1

Summary of 454 sequencing data
Species Number of loci1 Copies/1C2 Reads mapped Coverage3 Number of clusters
18S ITS1 18S ITS1 18S ITS1
N. tomentosiformis 1 1400 1190 1592 0.9 1.1 204 190
N. sylvestris 3 5100 2657 3882 0.5 0.8 339 483
N. otophora 2 1500 1469 2308 1.0 1.5 105 303
N. kawakamii 3+dispersed 2200 998 1093 0.5 0.5 138 213

1See Figure 6 and Plant rDNA database - http://www.plantrdnadatabase.com webcite[42].

2The gene copies were initially determined by slot blot hybridisation [43] and later adjusted by calculation from the NGS data [22] using a http://w3lamc.umbr.cas.cz/profrep/ webcite server. For N. kawakamii and N. otophora the copies were determined based on Southern blot signals (Figure 5).

3Mean read-depth: the number of aligned reads divided by rDNA copy number.

Matyášek et al.

Matyášek et al. BMC Genomics 2012 13:722   doi:10.1186/1471-2164-13-722

Open Data