(A) Structure of 35S rDNA unit in Nicotiana (redrawn from). The transcription start site is arrowed. IGS = intergenic spacer, ITS = internal transcribed spacer (B) PCR primer binding sites to the 18S and 5.8S rDNA unit and 454 sequencing reads generated. The total amplicon length was 650 bp.
Matyášek et al. BMC Genomics 2012 13:722 doi:10.1186/1471-2164-13-722