Email updates

Keep up to date with the latest news and content from BMC Genomics and BioMed Central.

Open Access Highly Accessed Research article

MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi

Evan K Maxwell12, Joseph F Ryan13, Christine E Schnitzler1, William E Browne4 and Andreas D Baxevanis1*

Author affiliations

1 Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA

2 Bioinformatics Program, Boston University, Boston, MA, 02215, USA

3 Sars International Center for Marine Molecular Biology, University of Bergen, Bergen, 5008, Norway

4 Department of Biology, University of Miami, Coral Gables, FL, 33146, USA

For all author emails, please log on.

Citation and License

BMC Genomics 2012, 13:714  doi:10.1186/1471-2164-13-714

Published: 20 December 2012

Abstract

Background

MicroRNAs play a vital role in the regulation of gene expression and have been identified in every animal with a sequenced genome examined thus far, except for the placozoan Trichoplax. The genomic repertoires of metazoan microRNAs have become increasingly endorsed as phylogenetic characters and drivers of biological complexity.

Results

In this study, we report the first investigation of microRNAs in a species from the phylum Ctenophora. We use short RNA sequencing and the assembled genome of the lobate ctenophore Mnemiopsis leidyi to show that this species appears to lack any recognizable microRNAs, as well as the nuclear proteins Drosha and Pasha, which are critical to canonical microRNA biogenesis. This finding represents the first reported case of a metazoan lacking a Drosha protein.

Conclusions

Recent phylogenomic analyses suggest that Mnemiopsis may be the earliest branching metazoan lineage. If this is true, then the origins of canonical microRNA biogenesis and microRNA-mediated gene regulation may postdate the last common metazoan ancestor. Alternatively, canonical microRNA functionality may have been lost independently in the lineages leading to both Mnemiopsis and the placozoan Trichoplax, suggesting that microRNA functionality was not critical until much later in metazoan evolution.

Keywords:
Mnemiopsis leidyi; Ctenophore; Metazoa; microRNA; miRNA; Drosha; Pasha; Microprocessor complex; Ribonuclease III; RNase III