Characterization of SCGB1A1 genomic sequences. (A) A partial SCGB1A1 genomic sequence was amplified by nested PCR using purified LR-PCR products as template. Primers (blue triangles) were designed to target SCGB1A1 exon 2 to 3 (green boxes), coding for the complete mature secreted protein. (B) Multiple sequence alignment was performed using the nested PCR products from SCGB1A1P, SCGB1A1 and SCGB1A1A. Samples from 24 animals were analyzed to establish the SCGB1A1P consensus sequence, and 12 samples for SCGB1A1 and SCGB1A1A. (C) A region of the SCGB1A1 exon 2 gene sequence showing the three individual single nucleotides (arrows) at positions 2217/2242/2284 (cDNA positions 150/175/217) used as internal markers for subsequent gene-specific identification (SCGB1A1P = C/A--G--A; SCGB1A1 = A--A--A; SCGB1A1A = A--G--G).
Côté et al. BMC Genomics 2012 13:712 doi:10.1186/1471-2164-13-712