qPCR validation of the microarray results. Genes expressed in increased levels in DA (six genes, black bars) and genes expressed in increased levels in DA.F344(Cia5a) congenics (three genes, grey bars) were selected for qPCR confirmation. Fold-differences were log-transformed. The same RNAs used in the microarray experiments were used for qPCR. ΔCt was used for statistical analyses; all genes had p≤0.04, t-test.
Brenner and Gulko BMC Genomics 2012 13:710 doi:10.1186/1471-2164-13-710