Genomic conservation falls after the PAS. Panel A. PhyloP conservation values for the genomic region spanning the first exon and first intron of a RefSeq annotated gene (GAPDH) (upper panel) and the last intron and the last exon of the same gene (lower panel). The windows were adapted from the UCSC genome browser . TSS, Transcriptional Start Site; ATG, start codon; stop, stop codon; PAS, polyadenylation site; T, transcribed interval encoding for the mature transcript; NT, interval not encoding for the mature transcript; CDI, Conservation Drop Index. The black arrows indicate the direction of transcription of the gene. Panel B. Absolute frequency of TSS and PAS annotated in the RefSeq database according to their CDI.
Morgan et al. BMC Genomics 2012 13:708 doi:10.1186/1471-2164-13-708