RT-PCR analysis (A) and plate growth assay (B) of N402 and B36. A, RT-PCR analysis of expression and transcript processing of the UPR transcription factor gene, hacA. The ratio between unspliced, a (220 bp), and spliced, b (200 bp), of hacA transcript is similar in all three N402 steady states of maltose-limited chemostat cultures while in B36 there is more spliced hacA present. The H2B control shows that there is no contamination with genomic DNA; genomic DNA,181-bp amplicons; mRNA, 131-bp amplicons. B, plate growth assay of N402 and B36 using different carbon sources. 104 spores were point-inoculated on MM plates and incubated for 4 days at 30°C.
Kwon et al. BMC Genomics 2012 13:701 doi:10.1186/1471-2164-13-701