Table 1

Conservation of active site residues of C. glutamicum (2S,3S)-2,3-butanediol dehydrogenase in Pcar_0903, Pcar_2068 and Ppro_3110, compared to meso-2,3-butanediol dehydrogenase of K. pneumoniae
C. glutamicum K. pneumoniae Pcar_0903 BudY Pcar_2068 BudZ Ppro_3110
S141 S139 S151 C144 S148
I142 Q140 I152 I145 I149
A143 A141 A153 A146 A150
F148 N146 F158 L151 F155
Y154 Y152 Y164 Y157 Y161
P184 P182 P194 P187 P191
G185 G183 G195 G188 G192
I186 I184 I196 I189 I193
M191 M189 M201 M194 M198
W192 W190 W202 W195 W199
I195 I193 I205 I198 I202

Mutations that could alter the substrate specificity from (2S,3S)-2,3-butanediol to meso-2,3-butanediol are indicated in italics.

Aklujkar et al.

Aklujkar et al. BMC Genomics 2012 13:690   doi:10.1186/1471-2164-13-690

Open Data