RT-PCR assessment of purity of Eimeria tenella merozoites, gametocytes and oocysts. Total RNA was extracted from E. tenella merozoite (M), gametocyte (G), unsporulated oocyst (UO) and sporulated oocyst (SO) preparations. cDNA was synthesized and stage-specific parasite genes (GAM56 for gametocytes; TFP250 for merozoites and sporozoites) was amplified by RT-PCR. PCR samples were run alongside chicken host cDNA (H), E. tenella genomic DNA (gD) and a negative control sample with no template (−) on a 1% agarose gel. The E. tenella β-actin gene product was used to standardize loading of parasite cDNA samples.
Katrib et al. BMC Genomics 2012 13:685 doi:10.1186/1471-2164-13-685