Table 1

Results of culture, nested PCR and LAMP analysis for three pigs challenged with H. parasuis serovar 5, presented as the number of pigs positive/pigs samples

Samples

H. parasuis culturea

Nested PCR

LAMP


Brain

0/3

1/3

1/3

Lymph node

3/3

3/3

3/3

Tonsil

2/3

2/3

2/3

Lung

3/3

3/3

3/3

Pericardial fluid

2/3

2/3

2/3

Heart

1/3

1/3

1/3

Spleen

3/3

3/3

3/3

Total site

14/21

15/21

15/21

%positive

67

71

71


a: if H. parasuis was not identified by culture due to contaminated overgrowth, presence of H. parasuis was checked by nested PCR and LAMP on a colony sweep.

Wang et al. BMC Genomics 2012 13:68   doi:10.1186/1471-2164-13-68

Open Data