Validation of mRNA and miRNA profiles using quantitative RT-PCR on the same sample set of microarray study. A. mRNA profiles validation. B. miRNA profiles validation. Data was analysed using the 2-ΔΔCt method and results were plotted as fold differences of relative expression normalized to house keeping gene (GAPDH) and Hs_RNU6B_3 individually. The data have been collected from the same RNA samples from which mRNA and miRNA profiling have been done. All the data presented in this figure have significant P value of <0.05 as calculated by Student's t-test.
Zhou et al. BMC Genomics 2012 13:677 doi:10.1186/1471-2164-13-677