Additional file 3.

Graph showing B. bacteriovorus Tiberius host-independent growth of cells from a predatorily grown culture: Spontaneously streptomycin-resistant Tiberius cells were taken directly from a culture grown on prey cells. They were then directly grown in PY broth (Horowitz et al., 1974) in the presence of streptomycin to inhibit growth of prey E. coli (still present in the co-culture). The resulting Tiberius growth was measured as an increase in OD at 600 nm, shown in the graph against time of incubation at 29°C. The larger prey-independent cells of Tiberius were large enough to measure by optical density (although attack phase sized Bdellovibrio are not) , thus the OD600nm values are probably an under-estimate of the growth rate of total Bdellovibrio (including smaller cells) in the population. The data shown are from 3 independent repeats and the error bars are 1 standard deviation from the mean.

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Hobley et al. BMC Genomics 2012 13:670   doi:10.1186/1471-2164-13-670