Distribution and assignment of 10,158 EST contigs derived from pyrosequencing of the pea-Sclerotinia sclerotiorum transcriptome using the tBLASTx method. EST contigs with an e-value score <1e-3 to only plant or fungal EST reference databases were automatically assigned. Those EST contigs with hits (<1e-3) to both databases were assigned based on a cutoff ratio of e-values (fungi/plant), those ESTs with a ratio > =1e20 were classified as pea, those < =1e-20 as Sclerotinia and those between >1e-20 and <1e20 as ambiguous. ESTs without any hits (>1e-3) were classified as unassigned ESTs.
Zhuang et al. BMC Genomics 2012 13:668 doi:10.1186/1471-2164-13-668