Integrated miRNA, mRNA and protein expression analysis reveals the role of post-transcriptional regulation in controlling CHO cell growth rate
- Equal contributors
1 National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland
2 Molecular Therapeutics for Cancer Ireland, Dublin City University, Dublin 9, Ireland
3 Bioprocess R&D, Pfizer Inc., Andover, Massachusetts, USA
BMC Genomics 2012, 13:656 doi:10.1186/1471-2164-13-656Published: 21 November 2012
Additional file 1:
Sample Information. Growth rates and " fast", "slow" designations for samples subjected to miRNA, mRNA and proteomic profiling.
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Additional file 2:
MicroRNA differential expression. DE miRNAs identified following TLDA analysis. Also included are the PCCs utilised to identify 51 high priority miRNAs.
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Additional file 3:
Protein differential expression. DE proteins identified using LC-MS/MS analysis and the BB-CHO proteomic database.
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Additional file 4:
mRNA differential expression. DE mRNA identified using the CHO specific WyeHamster3a Affymetrix microarray.
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Additional file 5:
GO enrichment analysis. Enrichment analysis of DE protein and mRNA lists against GO biological processes using DAVID.
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Additional file 6:
in-silico miRNA target prediction of Group A targets. Identification of potential targets using TargetScan for candidate Group A.
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Additional file 7:
in-silico miRNA target prediction of Group B targets. Identification of potential targets using TargetScan for candidate Group B.
Format: XLSX Size: 47KB Download file