Overview of RNA sample preparation. Whole brains were dissected from isofemale lines derived from a European (the Netherlands) and an African (Zimbabwe) population. Brains were pooled within each population for RNA extraction. The dissection and pooling procedures were performed for two biological replicates of each population and sex, resulting in a total of eight samples that were used for cDNA library construction and high-throughput sequencing.
Catalán et al. BMC Genomics 2012 13:654 doi:10.1186/1471-2164-13-654