Comparison of unigene expression among different DGE samples. The gene expression level was calculated by using RPKM method (Reads Per kb per Million reads). As the RPKM method can eliminate the influence of different gene length and sequencing discrepancy on the calculation of gene expression, the calculated gene expression can be directly used for comparing the difference of gene expression among samples. The expression of unigenes in response to NNV infection was analyzed by comparing the RPKM of unigenes in the infected versus non-infected GK cells at 6 (A) and 33 (B) hours post-infection. NNV: NNV-infected GK cells, Control: non-infected GK cells.
Lu et al. BMC Genomics 2012 13:651 doi:10.1186/1471-2164-13-651