Validation of microarray data by real-time qRT-PCR for selected C. parvum genes. (A) Relative mRNA levels of C. parvum genes in the oocyst stage. Microarray signals are shown as the means of normalized signals derived from all probes in all 8 arrays (left axis). Levels determined by semi-qRT-PCR are displayed as the fold differences in relative to the mean of all samples in at least three biological replicates (right axis). (B) Fold changes of the gene expressions in C. parvum oocysts recovered for 30 min after UV-treatment. Bars indicate standard deviations (SDs).
Zhang et al. BMC Genomics 2012 13:647 doi:10.1186/1471-2164-13-647