Table 3

ChIP-seq from primary cells isolated from human monozygotic twins
Twin Pair Twin pair 1 Twin pair 2 Twin pair 3
CD4+cells
Cell no. / IP 5 x 105 5 x 105 5 x 105 5 x 105 5 x 105 5 x 105
No. reads 35 244 517 44 255 574 40 644 508 45 738 891 38 332 819 29 484 478
No. unique, nonduplicate reads 3 978 339 6 524 094 3 316 312 3 340 926 3 130 804 1 167 824
No. Peaks called 14 828 12 622 15 598 15 731 15 825 13 719
No. overlapping peaks (%) 12 457 (82%) 14 833 (94%) 13 091 (83%)
CD8+cells
Cell no. / IP 4.3 x 105 3.7 x 105 4.7 x 105 5 x 105 3.8 x 105 4.2 x 105
No. reads 45 245 361 35 681 254 38 455 042 35 257 788 34 505 357 41 729 689
No. unique, nonduplicate reads 18 996 309 4 778 486 12 403 566 6 654 203 5 840 308 4 438 574
No. Peaks called 17 704 18 720 20 728 18 145 18 743 20 899
No. overlapping peaks (%) 14 578 (78%) 18 828 (76%) 15 312 (73%)

Peaks were called using MACS, allowing no ambiguously mapping or duplicate reads. Peaks with p-values > 1x10-10 were excluded from analysis. Numbers of overlapping peaks were counted, and expressed as a percentage of the highest peak count for the twin pair.

Gilfillan et al.

Gilfillan et al. BMC Genomics 2012 13:645   doi:10.1186/1471-2164-13-645

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