Transcriptional and metabolomic analysis of Ascophyllum nodosum mediated freezing tolerance in Arabidopsis thaliana
1 Department of Environmental Sciences, Dalhousie Agricultural Campus, Dalhousie University, Truro, NS, B2N 5E3, Canada
2 Institute for Nutrisciences and Health, National Research Council of Canada, Charlottetown, PEI, C1A 4P3, Canada
3 Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 550 University Avenue, Charlottetown, PE, C1A 4N6, Canada
4 Atlantic Food and Horticulture Research Centre, Agriculture and Agri-Food Canada, Kentville, NS, B4N 1J5, Canada
5 Acadian Seaplants Limited, 30 Brown Ave., Dartmouth, NS, B3B 1X8, Canada
BMC Genomics 2012, 13:643 doi:10.1186/1471-2164-13-643Published: 21 November 2012
Additional file 1:
Figure S1. Global gene expression of Arabidopsis plants treated with lipophilic fraction of ANE (LPC) during freezing stress and post freezing recovery period. Volcano plot of differential expression pattern during (a) freezing stress and (b) post freezing recovery with ≥1.5 foldchange and P≤0.05 cut off. Scatter plot of log2 signal intensities of three replicate samples from LPC-treated plants during (c) freezing stress and (d) post freezing recovery. Figure S2. Functional categorization of differentially expressed genes. Normalized frequency (Provart and Zhu, 2003) categories of (a) up, or (c) down-regulated genes in LPC-treated plants during freezing. (b&d) functional categorization (b) up or (d) down-regulated genes during freezing according to the actual number of genes. Figure S3. Functional categorization of differentially expressed genes. Normalized frequency (Provart and Zhu, 2003) categories of (a) up, or (c) down-regulated genes in LPC-treated plants during post-freezing recovery period. (b& d) functional categorization (b) up or (d) down-regulated genes according to the actual number of genes during post-freezing recovery period. Figure S4. Heat map of selected differentially expressed genes in LPC treated plants during (FR) freezing stress and (TH) post freezing recovery period. Expression-level change is shown in a color relative to the expression level, as indicated in the color scale bar.
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Additional file 2:
Table S1. Primer sets and the PCR conditions used for gene expression studies. This material is available as part of the online article from: http://www.blackwell-synergy.com/doi/ webcite. Table S2. A list of common genes which showed the similar pattern of expression (either up-regulated, or down-regulated) during freezing and post-freezing thawing period. Table S3. List of genes selected for RT-PCR confirmation of microarray results.
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