Table 1

List of the 18 spots identified by LC-ESI-MS/MS whose concentration is modulated by leaf rust infection in barley leaves
Spot ID Accession number Species Protein description EC Abb. Mra/pIa Mrb/ pIb Cov. (%) c
Photosynthesis and energy metabolism
2577 Q40073 Hordeum vulgare Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic RuACS (b) 40.2 / 5.3 46.1 / 5.6 e 43.5 e
2581 Q40073 Hordeum vulgare Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic RuACS (c) 40.8 / 5.2 46.1 / 5.6 e 34.6 e
2760 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 25.4 / 6.4 52.9 / 6.2 e 16.3 e
2776 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 24.2 / 6.4 52.9 / 6.2 e 22.6 e
2866 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 22.3 / 5.2 52.9 / 6.2 e 8.0 e
2952 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 21.3 / 5.1 52.9 / 6.2 e 9.0 e
2983 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 20.1 / 5.3 52.9 / 6.2 e 4.6 e
3004 XP_002465461 Sorghum bicolor ATP synthase β chaind ATPase β 19.4 / 4.1 22.8 / 5.3 6.1
3009 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 19.2 / 5.2 52.9 / 6.2 e 4.2 e
3118 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 19.7 / 5.0 52.9 / 6.2 e 6.9 e
3142 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 20.4 / 4.7 52.9 / 6.2 e 4.4 e
3150 P05698 Hordeum vulgare Ribulose bisphosphate carboxylase large chain 4.1.1.39 RuLC 20.7 / 4.8 52.9 / 6.2 e 4.8 e
Carbohydrate metabolism
2333 CAZ64535 Hordeum vulgare Sucrose synthase 2.4.1.13 SuSy 88.2 /5.9 92.2 / 5.8 18.4
2962 CAC32847 Hordeum vulgare Adenosine diphosphate glucose pyrophosphatase AGPPase 21.0 / 5.1 19.5 / 5.7 e 17.4 e
Protein degradation
2449 XP_002454700 Sorghum bicolor Leucine aminopeptidase 2,chloroplasticd 3.4.11.1 LAP 2 57.0 / 5.6 61.8 / 7.6 20.0
Defence responses
2886 CAA55345 Hordeum vulgare chitinase 3.2.1.14 CHI 22.3 / 5.2 26.6 / 6.1 29.4
2956 BAD31057 Oryza sativa Chitinase III-like protein CHI III 21.2 / 5.3 18.9 / 6.5 12.7
Secondary metabolism
2613 CAA54616 Hordeum vulgare Flavonoid 7-O-methyltransferase 2.1.1.6 F-OMT 39.2 / 5.4 42.3 / 5.4 29.5

Statistical information about LC-ESI-MS/MS analysis are reported in Additional files 1 and 2.

Abb.: abbreviation.

a: experimental molecular weight and pI.

b: theoretical molecular weight and pI.

c: amino acid coverage (%).

d: annotation obtained by BLAST-P alignment analysis against the Viridiplantae subset of the nr-database at NCBI.

e: value referred to the mature form of the protein.

Bernardo et al.

Bernardo et al. BMC Genomics 2012 13:642   doi:10.1186/1471-2164-13-642

Open Data