Spread of B. cenocepacia and host cytotoxicity in an unchecked infection of murine macrophages. Capacity to spread (A) is calculated as the fold-increase in infected macrophages when maintained in antibiotic-free media rather than 10 μg/ml gentamicin in the same experiment. Host cytotoxicity (B) is calculated as the ratio of total dead cells to infected cells. 50 000 cells were counted in each of three independent experiments. Standard error bars are indicated. Significance was determined using one-way ANOVA and Dunnett's Multiple Comparison Test. Mutants demonstrating significant difference from the wild-type are indicated (*p < 0.05, **p < 0.01, ***p < 0.001).
Tolman and Valvano BMC Genomics 2012 13:63 doi:10.1186/1471-2164-13-63