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Open Access Research article

Global changes in gene expression by the opportunistic pathogen Burkholderia cenocepacia in response to internalization by murine macrophages

Jennifer S Tolman1 and Miguel A Valvano12*

Author Affiliations

1 Infectious Diseases Research Group, Department of Microbiology & Immunology, University of Western Ontario, London, Ontario, N6A 5C1, Canada

2 Department of Medicine, University of Western Ontario, London, Ontario, N6A 5C1, Canada

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BMC Genomics 2012, 13:63  doi:10.1186/1471-2164-13-63

Published: 9 February 2012

Additional files

Additional file 1:

Figure S1-Bacterial transcripts specific to intracellular B. cenocepacia can be identified by differential hybridization.

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Additional file 2:

Table S1-Intracellular-specific selectively captured sequences.

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Additional file 3:

Table S2-Genes with significantly higher or lower expression by intracellular B. cenocepacia. Genes included show greater than 2-fold change in expression (p < 0.05) in intracellular bacteria relative to non-macrophage-exposed bacteria. The first sheet contains genes with increased intracellular expression, the second genes with decreased intracellular expression.

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Additional file 4:

Table S3-In vivo essential genes with increased expression in intracellular bacteria.

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Additional file 5:

Figure S2-Fold change gene expression of selected SCOTS-identified genes measured by qRT-PCR.

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Additional file 6:

Figure S3-ΔBCAL0124 and ΔBCAM2837 are defective in motility.

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Additional file 7:

Figure S4-Flagellin is necessary for efficient bacterial entry into macrophages.

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Additional file 8:

Table S4-Oligonucleotide primers used in this study.

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