Additional file 4.

Hierarchical clustering of putative secreted proteins identified in Anophelessalivary gland extracts. Proteins from An. gambiae, An. arabiensis, An. stephensi and An. albimanus SGEs were identified by mass spectrometry after in-gel trypsin digestion. Protein sequences were submitted to SignalP 3.0 server [65] to select putative secreted proteins and were hierarchically clustered at ≥ 90%, ≥ 70% and ≥ 40% identity threshold with CD-HIT web server [68]. * indicate the representative (i.e., longest) protein sequence of each cluster. Anopheles species in which secreted salivary proteins were identified are indicated. The last common taxon encompassing homologous proteins at the genus level is indicated according to in silico results (q.v. Additional file 2). n.a.: non-available (i.e., uncharacterized protein sequences that were not recovered in the in silico analysis). Lines in bold indicate proteins identified in antigenic bands (Figure 5A, Additional file 5). AGA, An. gambiae; AGA#, An. gambiae PEST strain (Pink Eye STandard); AAR, An. arabiensis; AST, An. stephensi; AAL, An. albimanus; MW: Molecular weight.

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Fontaine et al. BMC Genomics 2012 13:614   doi:10.1186/1471-2164-13-614