Table 5

Heat-shocked and regenerated plants from different HSP::TRANSPOSASE/ATDs double transgenic aspen lines without any phenotypic alterations (anonymous approach) grown in the greenhouse were randomly selected and tested for ATDs transposition with the primer pair 16/37.

Transgenic line

Tested plants

PCR-positive (16/37) (%)

TAIL-PCR and positive BLAST hits

Transcript annotation


N82-2

22

3

0

---


N82-5

26

11

5

3


N82-7

24

4

0

---


N82-11

20

4

1

0


N82-14

13

4

2

0


N82-15

25

14

8

6


N92-1

19

16

6

4


N92-2

19

10

0

---


N92-3

20

0

0

---


N92-4

10

6

1

0


N95-1

23

2

0

---


N95-2

19

2

0

---


N95-3

19

9

1

1


N95-4

10

0

0

---


N95-5

10

0

0

---


N95-6

21

10

2

1


SUM (%)

300

95 (32%)

26 (8.7%)

15 (5.0%)


Positive candidates were subjected to TAIL-PCR and sequencing to determine the new ATDs genomic insertion locus. Obtained sequences were blasted against the publicly available genome sequence of P. trichocarpa v2.0 (Phytozome v7.0; http://www.phytozome.net/poplar webcite). Numbers of positive BLAST hits and, if applicable, of transcript annotations are given.

In bold the three lines with highest numbers of positive ATDs transposition and BLAST hits.

Fladung and Polak BMC Genomics 2012 13:61   doi:10.1186/1471-2164-13-61

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