Table 4 |
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|
Pronounced phenotypes of ATDs tagged poplar lines: annotation results of new ATDs flanking sequences. |
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|
Transgenic line |
Number of variants |
Variant affiliation |
Phenotype |
Genomic insertion locus (scaffold:position)* |
Score |
E-value |
Transcript (POPTR_) |
Functional annotation |
|
|
||||||||
|
N82-2-11 |
Variegated leaf |
n.d.* |
||||||
|
|
||||||||
|
N82-2-13 |
Variegated leaf |
18:5,952,981 |
154.6 |
1.5e-35 |
||||
|
|
||||||||
|
N82-2 |
5 |
N82-2-14 |
Dwarf plant, weakly shriveled leaf |
18:5,946,821 |
253.8 |
3.6e-66 |
0018s06220 |
2OG-Fe(II) oxygenase superfamily |
|
|
||||||||
|
N82-2-64 |
Shriveled leaf |
1599:3,445 |
250.2 |
3.8e-65 |
||||
|
|
||||||||
|
N82-2-70 |
Wavy leaf |
3:15,139,843 |
702.8 |
0 |
0003s15320 |
Hypothetical protein (basic region leucine zipper) |
||
|
|
||||||||
|
N82-3-23 |
Serrated leaf |
10:20,130,280 |
206.9 |
5.7e-52 |
0010s23600 |
1,4-alpha-glucan branching enzyme/starch branching enzyme II |
||
|
|
||||||||
|
N82-3 |
3 |
N82-3-37 |
Serrated leaf |
18:8,430,672 or |
1063.5 |
0 |
0018s07730 |
No functional annotation for this gene |
|
|
||||||||
|
15:1,812,282 |
1059.9 |
0 |
0015s02530 |
Myosin VII |
||||
|
|
||||||||
|
N82-3-66 |
Shriveled leaf |
7:3,447,049 |
311.5 |
9.1e-83 |
0007s05350 |
Histone binding protein RBBP4 |
||
|
|
||||||||
|
N82-5-3 |
Lanceolated leaf |
14:11,531,333 |
450.3 |
1.6e-124 |
||||
|
|
||||||||
|
N82-5 |
4 |
N82-5-20 |
Necrotic leaf |
14:2,480,856 |
302.4 |
9.4e-81 |
||
|
|
||||||||
|
N82-5-26 |
Bonsai plant |
n.d.* |
||||||
|
|
||||||||
|
N82-5-28 |
Bonsai plant |
607:2,304 |
448.5 |
3.7e-124 |
0607s00200 |
No functional annotation for this gene |
||
|
|
||||||||
|
N82-7 |
1 |
N82-7-1 |
Shriveled leaf |
11:13,627,432 |
565.7 |
1.7e-159 |
0011s10950 |
Protein of unknown function (DUF_B2219) |
|
|
||||||||
|
N82-11-1 |
Crippled growth in vitro, saw toothed leaf |
16:3,641,919 |
280.8 |
1.5e-73 |
0016s05700 |
Zinc ion binding; nucleic acid binding |
||
|
|
||||||||
|
N82-11 |
3 |
N82-11-4 |
Pale green leaf |
n.d.* |
||||
|
|
||||||||
|
N82-11-5 |
Pale green leaf |
16:13,607,077 |
426.9 |
1.4e-117 |
0016s14360 |
EF-P Elongation factor |
||
|
|
||||||||
|
N82-14-2 |
Lanceolated leaf |
4:23,008,963 or |
780.3 |
0 |
0004s24320 |
Protein of unknown function (DUF1218) |
||
|
|
||||||||
|
17:944,412 |
1115.8 |
0 |
0017s01360 |
Apoptotic ATPase |
||||
|
|
||||||||
|
N82-14-3 |
Shriveled leaf |
14:6,540,903 |
904.8 |
0 |
0014s08850 |
Glycosyl hydrolases family 18; Pt-CHI3.5 |
||
|
|
||||||||
|
N82-14-4 |
Weakly serrated leaf |
11:1,379,364 or |
535.1 |
1.2e-150 |
||||
|
|
||||||||
|
N82-14 |
7 |
3:13,395,264 |
1079.7 |
0 |
0003s12970 |
No functional annotation for this gene |
||
|
|
||||||||
|
N82-14-5 |
Shriveled leaf |
10:14,732,583 |
892.2 |
0 |
0010s15550 |
No functional annotation for this gene |
||
|
|
||||||||
|
N82-14-6 |
Weakly shriveled leaf |
2:13,782,413 |
298.8 |
1.1e-79 |
||||
|
|
||||||||
|
N82-14-9 |
Shriveled leaf |
14:10,351,781 |
1090.5 |
0 |
0014s14140 |
Protein of unknown function (DUF3754) |
||
|
|
||||||||
|
N82-14-10 |
Undulating leaf |
17:1,256,405 |
1204.1 |
0 |
||||
|
|
||||||||
|
N82-15-6 |
Bushy plant |
4:9,615,348 |
1577.4 |
0 |
0004s11010 |
Pentatricopeptide repeat-containing protein |
||
|
|
||||||||
|
N82-15 |
2 |
N82-15-10 |
Lanceolate, serrated |
17:2,279,602 or |
226.7 |
5.9e-58 |
0017s03150 |
No functional annotation for this locus |
|
|
||||||||
|
leaf |
14:14,660,942 |
226.7 |
5.2e-58 |
|||||
|
|
||||||||
|
N92-1 |
2 |
N92-1-1 |
Variegated leaf |
10:15,124,072 |
147.4 |
6.0e-34 |
0010s16070 |
Stress responsive A/B Barrel Domain |
|
|
||||||||
|
N92-1-6 |
Variegated leaf |
10:15,114,170 |
206.9 |
1.2e-51 |
||||
|
|
||||||||
|
N95-1 |
1 |
N95-1-1 |
Variegated leaf |
n.d.* |
||||
|
|
||||||||
|
N95-3 |
1 |
N95-3-1 |
Variegated leaf |
n.d.* |
||||
|
|
||||||||
|
Genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript. In BLAST-analyses where more than one hit was given, either the one with lower e-value or when similar both hits are shown. *n.d. = not determined. |
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|
Fladung and Polak BMC Genomics 2012 13:61 doi:10.1186/1471-2164-13-61 |
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