Table 4

Pronounced phenotypes of ATDs tagged poplar lines: annotation results of new ATDs flanking sequences.

Transgenic line

Number of variants

Variant affiliation

Phenotype

Genomic insertion locus (scaffold:position)*

Score

E-value

Transcript (POPTR_)

Functional annotation


N82-2-11

Variegated leaf

n.d.*


N82-2-13

Variegated leaf

18:5,952,981

154.6

1.5e-35


N82-2

5

N82-2-14

Dwarf plant, weakly shriveled leaf

18:5,946,821

253.8

3.6e-66

0018s06220

2OG-Fe(II) oxygenase superfamily


N82-2-64

Shriveled leaf

1599:3,445

250.2

3.8e-65


N82-2-70

Wavy leaf

3:15,139,843

702.8

0

0003s15320

Hypothetical protein (basic region leucine zipper)


N82-3-23

Serrated leaf

10:20,130,280

206.9

5.7e-52

0010s23600

1,4-alpha-glucan branching enzyme/starch branching enzyme II


N82-3

3

N82-3-37

Serrated leaf

18:8,430,672 or

1063.5

0

0018s07730

No functional annotation for this gene


15:1,812,282

1059.9

0

0015s02530

Myosin VII


N82-3-66

Shriveled leaf

7:3,447,049

311.5

9.1e-83

0007s05350

Histone binding protein RBBP4


N82-5-3

Lanceolated leaf

14:11,531,333

450.3

1.6e-124


N82-5

4

N82-5-20

Necrotic leaf

14:2,480,856

302.4

9.4e-81


N82-5-26

Bonsai plant

n.d.*


N82-5-28

Bonsai plant

607:2,304

448.5

3.7e-124

0607s00200

No functional annotation for this gene


N82-7

1

N82-7-1

Shriveled leaf

11:13,627,432

565.7

1.7e-159

0011s10950

Protein of unknown function (DUF_B2219)


N82-11-1

Crippled growth in vitro, saw toothed leaf

16:3,641,919

280.8

1.5e-73

0016s05700

Zinc ion binding; nucleic acid binding


N82-11

3

N82-11-4

Pale green leaf

n.d.*


N82-11-5

Pale green leaf

16:13,607,077

426.9

1.4e-117

0016s14360

EF-P Elongation factor


N82-14-2

Lanceolated leaf

4:23,008,963 or

780.3

0

0004s24320

Protein of unknown function (DUF1218)


17:944,412

1115.8

0

0017s01360

Apoptotic ATPase


N82-14-3

Shriveled leaf

14:6,540,903

904.8

0

0014s08850

Glycosyl hydrolases family 18; Pt-CHI3.5


N82-14-4

Weakly serrated leaf

11:1,379,364 or

535.1

1.2e-150


N82-14

7

3:13,395,264

1079.7

0

0003s12970

No functional annotation for this gene


N82-14-5

Shriveled leaf

10:14,732,583

892.2

0

0010s15550

No functional annotation for this gene


N82-14-6

Weakly shriveled leaf

2:13,782,413

298.8

1.1e-79


N82-14-9

Shriveled leaf

14:10,351,781

1090.5

0

0014s14140

Protein of unknown function (DUF3754)


N82-14-10

Undulating leaf

17:1,256,405

1204.1

0


N82-15-6

Bushy plant

4:9,615,348

1577.4

0

0004s11010

Pentatricopeptide repeat-containing protein


N82-15

2

N82-15-10

Lanceolate, serrated

17:2,279,602 or

226.7

5.9e-58

0017s03150

No functional annotation for this locus


leaf

14:14,660,942

226.7

5.2e-58


N92-1

2

N92-1-1

Variegated leaf

10:15,124,072

147.4

6.0e-34

0010s16070

Stress responsive A/B Barrel Domain


N92-1-6

Variegated leaf

10:15,114,170

206.9

1.2e-51


N95-1

1

N95-1-1

Variegated leaf

n.d.*


N95-3

1

N95-3-1

Variegated leaf

n.d.*


Genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.

In BLAST-analyses where more than one hit was given, either the one with lower e-value or when similar both hits are shown.

*n.d. = not determined.

Fladung and Polak BMC Genomics 2012 13:61   doi:10.1186/1471-2164-13-61

Open Data