Southern blot analysis of transformed poplar lines carrying the plasmids p6-HSP-TP-OCS and p7N-ATDs-rolC. A representative blot with ScaI restricted and nptII-probed DNA isolated from Agrobacterium strain used for transformation, negative control line Esch5, and eleven transgenic lines N82-2 to -5, N82-7 to -8, N82-10 to -12, N82-14 to -15 is shown. ScaI has only one restriction site in the cassette that can be used for copy number determination. Genomic DNA was separated on agarose gel, blotted and hybridized with a DIG-labelled nptII probe. A.t.: positive control (Agrobacterium tumefaciens), Esch5: non-transformed control line.
Fladung and Polak BMC Genomics 2012 13:61 doi:10.1186/1471-2164-13-61