Surface Plasmon Resonance analysis of CBEL-glycoproteins interactions. Human lactotransferin (LTF) or melon hydroxyprolin-rich glycoprotein (HRGP) were passed over the CBEL chip surface at concentrations of 25 or 100 μg/ml respectively. Then, either HBS-Ep buffer alone (control) or monosaccharide solutions (GalNAc, Gal, Glc) were injected during the dissociation phase, 50 or 94 seconds after the end of LTF or HRGP injection respectively. The percentage of protein complex dissociation was measured 385 seconds after the beginning of buffer or monosaccharide injection. Data are the means of the values recorded on the two channels of the Biacore X flow cell ± SD.
Larroque et al. BMC Genomics 2012 13:605 doi:10.1186/1471-2164-13-605