Open Access Methodology article

A model system for assessing and comparing the ability of exon microarray and tag sequencing to detect genes specific for malignant B-cells

Maria Bro Kloster1, Anders Ellern Bilgrau12, Maria Rodrigo-Domingo12, Kim Steve Bergkvist1, Alexander Schmitz1, Mads Sønderkær3, Julie Støve Bødker1, Steffen Falgreen1, Mette Nyegaard1, Hans Erik Johnsen1, Kåre Lehmann Nielsen3, Karen Dybkaer1 and Martin Bøgsted1*

Author Affiliations

1 Department of Haematology, Aalborg Hospital, Aarhus University Hospital, Sdr. Skovvej 15, 9000, Aalborg, Denmark

2 Department of Mathematical Sciences, Aalborg University, Fredrik Bajers Vej 7G, 9220, Aalborg Ø, Denmark

3 Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngårdsholmsvej 49, 9000, Aalborg, Denmark

For all author emails, please log on.

BMC Genomics 2012, 13:596  doi:10.1186/1471-2164-13-596

Published: 5 November 2012

Additional files

Additional file 1:

Analysis workflow.

Format: PDF Size: 15KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Correspondence curve (solid blue line) and 5% acceptance region (grey area) for the test of independence in gene selection between the exon microarray and tag-seq platforms.

Format: PDF Size: 271KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Differentially expressed mRNA transcripts between the pure OCI-Ly8 and HEK293 samples identified by tag-seq.

Format: CSV Size: 20KB Download file

Open Data

Additional file 4:

Differentially expressed mRNA transcripts between the pure OCI-Ly8 and HEK293 samples identified by tag-seq.

Format: CSV Size: 6KB Download file

Open Data

Additional file 5:

Hierarchical clustering after subsampling of all cell populations based on all genes (A) exon microarray and (B) tag-seq.

Format: PDF Size: 13KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 6:

Model control for the linear, NB2 and NB1 models.

Format: PDF Size: 180KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 7:

Session information.

Format: TXT Size: 1KB Download file

Open Data