Expression analysis of all combinations of SNPs Ex 1–1, Ex 1–2 and Ex 11–1. (A) Illustration of eight haplotype constructs for UGDH. (B) Statistical analysis of real-time RT-PCR data for the eight plasmid constructs. 18sRNA was used to normalize expression of each construct. Compared with g-a-c, g-c-t, a-a-t, a-c-c, a-a-c, g-a-t, and a-c-t decrease the expression of UGDH, by 51.8%, 53.9%, 61.6%, 63%, 59.7% and 68.2%, respectively. (C) Representative Western blotting images for eight constructs of UGDH and β-Actin. (D) Statistical analysis of the protein level of eight constructs of UGDH normalized to β-Actin, which indicated a-a-t, a-c-c, a-a-c, g-c-c, and a-c-t similarly decreased UGDH protein expression, by 37.8%, 51.1%, 34.8%, 26.6%, and 50.1%, respectively, compared with the construct g-a-c. Values are given as means ± S.E.M. (* P <0.05, ** P <0.01; n = 5/group.
Xu et al. BMC Genomics 2012 13:590 doi:10.1186/1471-2164-13-590