Table 4

Distributions of lengths of cDNA clones completely sequenced by the primer walking and transposon shotgun sequencing methods
Length (bp) Sequenced using universal primersa Primer walking Transposon shotgun sequencing
Once Twice Total
–1000 1174 1956 93 2049 156
1001–1500 928 9062 913 9975 519
1501–2000 67 6201 1843 8044 1058
2001–2500 0 2817 731 3548 766
2501–3000 0 919 428 1347 450
3000–3500 0 48 294 342 286
3501–4000 0 1 85 86 165
4001– 0 0 19 19 100
Total 2169 21,004 4406 25,410 3500
Average (bp) 907.6 1548.3 1959.6 1620.0 2163.3

Lengths of cDNA clones are shown according to the sequencing methods used. Clones sequenced by using the primer walking (PW) method are shown separately according to the number of primer walkings. Clones sequenced by using transposon shotgun sequencing (TPS) include those that were finished by the TPS method after being sequenced by the PW method.

a Clones that were completely sequenced just by using universal primers (forward and reverse primers aligned to the vector sequence, and T25V primer).

Uenishi et al.

Uenishi et al. BMC Genomics 2012 13:581   doi:10.1186/1471-2164-13-581

Open Data