EMSA of the promoter region for several V. parahaemolyticus genes in the presence (+) or absence (-) of purified LexA protein from this organism. Competition assays (Additional file 3) were conducted to validate the specificity of LexA binding. The absence of purified LexA protein is used as a negative control for each EMSA. The lexA, recA and imuA promoter regions are used as positive controls.
Sanchez-Alberola et al. BMC Genomics 2012 13:58 doi:10.1186/1471-2164-13-58