Table 1

Differentially expressed genes in T cells polarized towards the Th0, Th1 and Th2 subsets
Top 18 Th0 specific genes Top 49 Th1 specific genes Top 50 Th2 specific genes
Affymetrix probe ID Gene symbol P(“Th0 specific”) Affymetrix probe ID Gene symbol P(“Th1 specific”) Affymetrix probe ID Gene symbol P(“Th2 specific”)
203881_s_at DMD(<2) 0.99967984 206618_at IL18R1(+) 0.999997088 204388_s_at MAOA(+) 1
223435_s_at PCDHA1(<2) 0.999832654 203881_s_at DMD(+) 0.999993101 227006_at PPP1R14A(+) 1
205027_s_at MAP3K8(<2) 0.996290375 210354_at IFNG(+) 0.999987382 205419_at GPR183(+) 1
228055_at NAPSB(<2) 0.996196899 207072_at IL18RAP(+) 0.9999811558 215172_at PTPN20A(+) 1
220225_at IRX4(<2) 0.991324709 213943_at TWIST1(+) 0.999952924 205769_AT SLC27A2(-) 1
205794_s_at NOVA1(<2) 0.984972756 222547_at MAP4K4(<2) 0.999933552 218976_at DNAJC12(-) 1
221035_s_at TEX14(<2) 0.981445323 242809_at IL1RL1(<2) 0.999876515 208510_s_at PPARG(+) 1
210354_at IFNG 0.977204551 206007_at PRG4(<2) 0.999828413 228708_at RAB27B(+) 1
212012_s_at KPNA6(<2) 0.976438534 228055_at NAPSB(+) 0.99973265 45288_at ABHD6(+) 1
219265_at MOBKL2B(<2) 0.974857762 235458_at HAVCR2(+) 0.99959007 229764_at TPGR1(+) 1
212992_at AHNAK2(<2) 0.971558242 225955_at METRIL(+) 0.99954195 235199_at RNF125(+) 1
223727_at KCNIP2(<2) 0.968952518 206974_at CXCR6(+) 0.999495469 203153_at IFIT1(-) 1
200907_s_at PALLD(<2) 0.967031439 206785_s_at KLRC2(<2) 0.99882105 203097_s_at RAPGEF2(-) 1
1570169_at CSMD2(<2) 0.950549266 200648_s_at GLUL(+) 0.997745987 208891_at DUSP6(+) 1
200906_s_at PALLD(<2) 0.940149574 205027_s_at MAP3K8(+) 0.996774094 223159_s_at NEK6(+) 1
216341_s_at GNRHR(<2) 0.933169413 225142_at JHDM1D(<2) 0.996653927 210715_s_at SPINT2(+) 1
222890_at CCDC113(<2) 0.921542966 215672_s_at AHCYL2(<2) 0.996237492 208158_s_at OSBPL1A(+) 1
201283_s_at TRAK1(<2) 0.914512473 219383_at PRR5L(<2) 0.995895316 225752_at NIPA1(+) 1
220603_s_at MCTP2(+) 0.993459782 206638_at HTR2B(+) 1
239533_at GPR155(<2) 0.992909201 205579_at HRH1(+) 1
229603_at BBS12(+) 0.989517632 226508_s_at TNFSF13B(-) 0.999999999
237559_at GPR55(<2) 0.988653639 244413_at CLECL1(+) 0.999999999
204284_at PPP1R3C(<2) 0.988109742 203708_at PDE4B(-) 0.999999999
202625_at LYN(<2) 0.986582272 227438_at ALPK1(-) 0.999999998
223767_at GPR84(<2) 0.984948052 210762_s_at DLC1(+) 0.999999998
209348_s_at MAF(+) 0.983885642 235570_at RBMS3(+) 0.999999997
210448_s_at P2RX5(+) 0.9830704 212077_at CALD1(+) 0.999999997
228057_at DDIT4L(<2) 0.978509028 235301_at KIAA1324L(+) 0.999999997
203129_s_at KIF5C(<2) 0.978078282 209576_at GNAI1(+) 0.999999997
1554190_s_at C10orf81(<2) 0.976976156 1554878_a_at ABCD3(+) 0.999999996
228806_at RORC(+) 0.976176574 205569_at LAMP3(+) 0.999999996
227568_at HECTD2(<2) 0.975089149 205900_at KRT1(+) 0.999999996
219753_at STAG3(+) 0.972731568 210145_at PLA2G4A(+) 0.999999996
223374_s_at B3GALNT1(<2) 0.971777376 227529_s_at AKAP12(+) 0.999999995
205098_at CCR1(<2) 0.969847411 209602_s_at GATA3(+) 0.999999995
200907_s_at PALLD(+) 0.967031845 225566_at NRP2(+) 0.999999995
211122_s_at CXCL11(<2) 0.965307014 1552807_a_at SIGLEC10(+) 0.999999995
227697_at SOCS3(+) 0.965003456 220307_at CD244(<2) 0.999999994
212683_at SLC23A44(<2) 0.960505919 239151_at BMS1P1(+) 0.999999994
213572_s_at SERPINB1(<2) 0.953874496 221241_s_at BCL214(-) 0.999999993
244321_at PGAP1(+) 0.953326576 229625_at GBP5(-) 0.999999992
217127_at CTH(+) 0.947970476 204912_at IL10RA(+) 0.999999999
225962_at ZNRF1(+) 0.944840091 214974_x_at CXCL5(-) 0.999999989
219532_at ELOVL4(<2) 0.937454137 221971_x_at CTGLF10P(+) 0.999999989
222838_at SLAMF7(+) 0.934337899 203853_s_at GAB2(+) 0.999999989
228658 MIAT(+) 0.934243107 222457_s_at LIMA1(+) 0.999999986
206948_s_at PMCH(<2) 0.928324396 213385_at CHN2(+) 0.999999983
232030_at KIAA1632(<2) 0.914780073 205630_at CRH(+) 0.999999979
213596_at CASP4(<2) 0.907680384 207861_at CCL22(+) 0.999999979
219209_at IFIH1(-) 0.999999977

Probe sets that fulfill the two-fold change criterion are marked based on the direction of the expression (+ denotes up-regulation and - denotes down-regulation) in the given condition. For example, IFNγ expression is enhanced in Th1 compared to Th0 and Th2, whereas expression of SLC27A2 is decreased in Th2 compared to Th0 and Th1. In addition, probe sets that do not fulfill the fold change criterion are marked in the lists with “<2”. All genes from Th0 and Th1 conditions as well as the top 50 of the Th2 specific genes are shown.

Äijö et al.

Äijö et al. BMC Genomics 2012 13:572   doi:10.1186/1471-2164-13-572

Open Data