Identification and characterization of microRNAs in white and brown alpaca skin
1 College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, People's Republic of China
2 Division of Animal and Nutritional Sciences, Laboratory of Animal Biotechnology and Genomics, West Virginia University, Morgantown, WV, 26506, USA
3 Laboratory of Mammalian Reproductive Biology and Genomics, Departments of Animal Science and Physiology, Michigan State University, East Lansing, MI, 48824, USA
BMC Genomics 2012, 13:555 doi:10.1186/1471-2164-13-555Published: 16 October 2012
Additional file 1:
Table S1. Heterogeneity at the 5’ and 3’ ends of the sequenced reads. The list of alpaca miRNAs different with miRNAs in miRBase based on sequencing counts.
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Additional file 2:
Table S2. Gene Ontology analysis of miRNAs targets. Targets of conserved miRNAs were grouped by Gene Ontology.
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Additional file 3:
Table S3. Novel miRNAs in white and brown alpaca skin. Sequences and MFE of candidate novel miRNAs in white and brown alpaca.
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Additional file 4:
Structure S4. Stem–loop structure of novel miRNAs. The stem-loop structure of 87 novel miRNAs in white alpaca and brown alpaca. The red underlined sequences are the predicted mature sequences.
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Additional file 5:
Table S5. miRNAs primers for real time PCR. List of miR-202, miR-211, miR-27a, miR-200a, miR-193b, miR-423 miR-484, miR-205, miR-137, miR-145 and U6 specific stem-loop RT primer, specific primers and universal primer.
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