Open Access Highly Accessed Research article

Identification and characterization of microRNAs in white and brown alpaca skin

Xue Tian1, Junbing Jiang1, Ruiwen Fan1, Haidong Wang1, Xiaolin Meng1, Xiaoyan He1, Junping He1, Hongquan Li1, Jianjun Geng1, Xiuju Yu1, Yunfei Song1, Danli Zhang1, Jianbo Yao12, George W Smith13 and Changsheng Dong1*

Author Affiliations

1 College of Animal Science and Technology, Shanxi Agricultural University, Taigu, 030801, People's Republic of China

2 Division of Animal and Nutritional Sciences, Laboratory of Animal Biotechnology and Genomics, West Virginia University, Morgantown, WV, 26506, USA

3 Laboratory of Mammalian Reproductive Biology and Genomics, Departments of Animal Science and Physiology, Michigan State University, East Lansing, MI, 48824, USA

For all author emails, please log on.

BMC Genomics 2012, 13:555  doi:10.1186/1471-2164-13-555

Published: 16 October 2012

Additional files

Additional file 1:

Table S1. Heterogeneity at the 5’ and 3’ ends of the sequenced reads. The list of alpaca miRNAs different with miRNAs in miRBase based on sequencing counts.

Format: XLSX Size: 17KB Download file

Open Data

Additional file 2:

Table S2. Gene Ontology analysis of miRNAs targets. Targets of conserved miRNAs were grouped by Gene Ontology.

Format: XLSX Size: 11KB Download file

Open Data

Additional file 3:

Table S3. Novel miRNAs in white and brown alpaca skin. Sequences and MFE of candidate novel miRNAs in white and brown alpaca.

Format: XLS Size: 31KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Structure S4. Stem–loop structure of novel miRNAs. The stem-loop structure of 87 novel miRNAs in white alpaca and brown alpaca. The red underlined sequences are the predicted mature sequences.

Format: PDF Size: 1.2MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5:

Table S5. miRNAs primers for real time PCR. List of miR-202, miR-211, miR-27a, miR-200a, miR-193b, miR-423 miR-484, miR-205, miR-137, miR-145 and U6 specific stem-loop RT primer, specific primers and universal primer.

Format: XLSX Size: 12KB Download file

Open Data