Table 3

miRNAs with predicted influence on SERCA2b expression
MicroRNA ΔG binding (Kcal mol-1) ΔG 70 nt flanking 3’ of predicted binding site (Kcal mol-1) ΔG 70 nt flanking 5’ of predicted binding site (Kcal mol-1) Predicted binding position in 3’-UTR SERCA2b Perfect SERCA2b complimentarity to “seed” region
miR-122 −24.9 −9.9 −8.7 313-334 no
−23.7 −3.0 −8.2 788-809 no
−21.5 −0.2 −9.5 496-517 no
−19.3 −8.2 −7.5 253-274 no
−19.3 −3.5 −5.2 476-497 no
−19.3 −6.3 −9.0 458-479 no
−18.1 −4.3 −8.3 151-172 no
miR-199a-3p −22.0 −4.9 −9.5 380-401 yes
−21.0 −3.1 −11.1 487-508 yes
−18.6 −8.3 −3.9 59-80 yes
−18.1 −7.5 −8.3 251-272 no
miR-320a −21.2 −9.9 −9.3 405-426 no
−21.8 −9.0 0.3 798-819 no
−21.2 −4.7 −9.8 65-86 partially (1–4)
−23.7 −8.2 −8.2 687-708 no
−21.8 −9.1 −11.9 123-144 partially (1–6)
miR-320b −21.2 −9.9 −9.3 405-426 no
−21.8 −9.0 0.3 798-819 no
−21.2 −4.7 −9.8 65-86 partially (1–4)
−23.7 −8.2 −8.2 687-708 no
−21.8 −9.1 −11.9 123-144 partially (1–6)
miR-320c −19.1 −8.0 0.3 800-819 no
−24.0 −12.4 −8.2 689-708 no
−21.1 −11.4 −9.2 650-669 no
miR-320d −23.4 −13.2 −8.2 690-708 no
−24.3 −12.2 −9.0 709-727 partially (1–4, 6–7)
miR-483-5p −23.8 −13.1 −9.0 705-726 no
−21.8 −4.7 −8.5 63-84 no
−22.7 −9.1 −9.3 121-142 no
−20.3 −8.2 −3.0 789-810 Partially (1, 3–6, 8)
miR-574-3p −22.9 −8.2 −6.5 347-368 No
−21.2 −2.7 −11.3 96-117 no
miR-574-5p −21.7 −8.6 −8.0 263-285 No
−23.4 −4.9 −3.5 479-501 No
−18.2 −3.3 −3.5 12-34 No
−20.5 −9.0 0.3 797-819 Partially (1–5, 7)
miR-140-3p −19.9 −8.2 −6.5 348-368 yes
−20.5 −9.9 −3.5 484-504 No
−19.7 −8.7 0.3 799-819 no

Boštjančič et al.

Boštjančič et al. BMC Genomics 2012 13:552   doi:10.1186/1471-2164-13-552

Open Data