Phylogenetic trees of representative fish species inhabiting divergent temperature realms. A: Sequence similarity based on the COI barcoding gene. B: Phylogenetic map based on the sequence information of the structural 16SrRNA sequence. Both trees were generated by using the neighbour joining method. Node distances are calculated with Tamura-Nei algorithm. Numbers indicate relative nucleotide substitutions in which different substitution pathways occur with independent probabilities. Sequence gaps were distributed proportional. A summary of the used sequence information is summarized in material and methods Table 3.
Windisch et al. BMC Genomics 2012 13:549 doi:10.1186/1471-2164-13-549