Additional file 3.

Figure S3. Sequence variation of the TcMUCII mucin gene family. Description: Entropy plots of the TcMUCII mucin gene family. TcMUCII mucin genes were extracted from Tcm, Tcc X10 and Tcc CLBR non-Esm. Sequences were aligned with ClustalW v2.1. Sequence entropy was calculated using the entropy function of the R package bio3d. Only alignment positions with less than 10% gaps were included in the analysis. The normalized entropy score was then plotted as a function of alignment position, where conserved sites (low entropy) score 1 and diverse (high entropy) sites score 0. The analysis indicated that 5′ and 3′ termini of TcMUCII mucin genes generally are the most conserved in all three genomes and that the central region is the most variable.

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Franzén et al. BMC Genomics 2012 13:531   doi:10.1186/1471-2164-13-531