Schematic overview of the sequence assembly. Schematic overview of the genome assembly steps. Illumina reads were assembled into contigs with Velvet. Unused reads were extracted and used for a second Velvet assembly with a different kmer length. 454 reads were assembled with CELERA. The 454-assembly was then subjected to homopolymer error correction with Illumina reads. The Illumina and 454 assemblies were merged into a non-redundant assembly using the Zorro pipeline. The assembly was then subjected to scaffolding using SSPACE and physical distance information. The final step involved gap closure with the IMAGE pipeline.
Franzén et al. BMC Genomics 2012 13:531 doi:10.1186/1471-2164-13-531