Open Access Research article

Annotated genetic linkage maps of Pinus pinaster Ait. from a Central Spain population using microsatellite and gene based markers

Marina de Miguel12, Nuria de Maria12, M Ángeles Guevara12, Luis Diaz12, Enrique Sáez-Laguna12, David Sánchez-Gómez1, Emilie Chancerel45, Ismael Aranda1, Carmen Collada23, Christophe Plomion45, José-Antonio Cabezas12 and María-Teresa Cervera12*

Author Affiliations

1 INIA-CIFOR, Departamento de Ecología y Genética Forestal, Carretera de la Coruña Km7.5, Madrid, 28040, Spain

2 Unidad Mixta de Genómica y Ecofisiología Forestal, INIA/UPM, Madrid, Spain

3 ETSIM, Departamento de Biotecnología, Ciudad Universitaria s/n, Madrid, 28040, Spain

4 INRA, UMR1202 BIOGECO, Cestas, F-33610, France

5 Université de Bordeaux, UMR1202 BIOGECO, Talence, 33170, France

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BMC Genomics 2012, 13:527  doi:10.1186/1471-2164-13-527

Published: 4 October 2012

Additional files

Additional file 1:

Sequence of contigs and genes containing the analyzed SNPs. Nucleotide sequences in fasta format of contigs and genes containing the polymorphic SNPs identified in the mapping progenies. All sequences are the consensus sequence obtained from different individuals except for genes LP3-1 and LP3-3 whit sequences obtained for one individual.

Format: FA Size: 162KB Download file

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Additional file 2:

Standard error of recombination frequency. Representation of the standard error of recombination frequency for markers segregating 1:1 in the F1 mapping populations C14xC15 (N=106) and C15xC14 (N=55). Standard error calculated following Ritter et al. [99].

Format: JPEG Size: 2.3MB Download file

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Additional file 3:

Polymorphic SNPs. List of the polymorphic SNPs in mapping progenies and associated features.

Format: XLSX Size: 33KB Download file

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Additional file 4:

Distorted markers. Number of total (assigned, ungrouped and excluded) markers showing distortion in their segregation rates at p ≤ 0.01 per LG obtained for C14 and C15 linkage maps constructed by merging two reciprocal crosses: C14xC15 and C15xC14.

Format: JPEG Size: 2.5MB Download file

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Additional file 5:

BLAST analysis of SNP-sequences. Results of BLAST-N search in pine databases of the Gene Index Project and GeneBank for mapped SNPs.

Format: XLSX Size: 31KB Download file

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Additional file 6:

GO annotation for mapped SNPs. Sequence distribution of GO terms for molecular function.

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Open Data