Resolution:
## Figure 3.
Impact of unequal sample sizes. Pairwise comparisons of perfect in silico replicate RNA-Seq datasets were made similarly
to Figure
2, but keeping the total read count for each pair constant while systematically varying
the relative size of both datasets, e.g., 1x10^{6} plus 9x10^{6}, 3 x10^{6} plus 7 x10^{6}, 5 x10^{6} plus 5 x10^{6}. Pearsonâ€™s r and Kappa fell for unequal sample sizes demonstrating a maximum for equal sample
sizes. SERE remained stable at 1.0 while the 99% CI of repeat measures was optimal
(smallest) for equal sample sizes.
Schulze |