Table 3

Pooling strategy for Illumina sequencing
Illumina sequencing technology Number of individuals perlane Number of lanes
GAII paired end 6 parents 1
GAII single end 16 progeny 6
HiSeq 2000 single end 24 progeny 3

Parents were sequenced with the GAII (paired end). Offspring were first sequenced with the GAII (single end); the depth of coverage was insufficient for genotyping and a second round of sequencing was done using the HiSeq 2000 (single end). The results of the GAII single end and HiSeq 2000 single end runs were filtered and trimmed to the same lengths and combined for analysis.

Everett et al.

Everett et al. BMC Genomics 2012 13:521   doi:10.1186/1471-2164-13-521

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