Open Access Highly Accessed Research article

Genome-wide metabolic (re-) annotation of Kluyveromyces lactis

Oscar Dias1, Andreas K Gombert2, Eugénio C Ferreira1 and Isabel Rocha1*

Author Affiliations

1 IBB – Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

2 Department of Chemical Engineering, University of Sao Paulo, Polytechnic School, PO Box 61548, 05424-970, Sao Paulo, SP, Brazil

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BMC Genomics 2012, 13:517  doi:10.1186/1471-2164-13-517

Published: 1 October 2012

Additional files

Additional file 1:

Figure S1. Merlin’s annotation interface. Figure S2. blast output format.

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Additional file 2:

Classification of Manual Curation Results.

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Additional file 3:

Table S1. K. lactis genes TCDB annotation. Table S2. Final annotation of the 2000 genes reviewed in this publication. Table S3. Comparison between the new annotation and KEGG’s annotation. Table S4. List of genes annotated as metabolic by KEGG but ruled out as metabolic in the new annotation. Table S5. Comparison between the new annotation and UniProt’s annotation. Table S6. Comparison between the new annotation and BRENDAS’s annotation. Table S7. List of genes assigned with metabolic functions by homology to organisms other that Saccharomyces cerevisiae in the new annotation. Table S8. Alphanumeric homologues classification. Table S9. Genes annotated using literature as main source. Table S10. List of genes assigned with transport functions, corresponding Superfamilies’ and families numbers. Table S11. KEGG Pathways in which new enzymes have been identified, by the new annotation. Table S12. Comparison between Ozier-Kalogeropoulos et.al. (1998) and the new annotation.

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Additional file 4:

GenBank files with Kluyveromyces lactis annotation Zip file with merlin generated GenBank Files.

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