Estimations of duck genome retention in the RH clones. A: retention frequencies of thirty-one microsatellite markers and four scaffold markers before (white) and after (grey) whole genome amplification. The test was done on the 90 selected hybrids by conventional Agarose genotyping. The expected chromosome locations of the markers (given in brackets) are derived from the chicken/duck comparative FISH mapping and a duck genetic map (Marie-Etancelin et al., in prep) for the microsatellite markers and according to comparative genomic data given by the Narcisse software  for the scaffold markers. B: Retention frequencies of thirty-nine scaffolds markers obtained using three different genotyping strategies. The thirty-nine scaffold markers were genotyped using either (i) the amplified panel with conventional agarose genotyping (blue: WGA-PCR), (ii) the non amplified panel and genotyping with the Fluidigm BioMark gene expression dynamic array (green: Pre-ampFLDMqPCR) or (iii) the amplified panel and genotyping with the Fluidigm BioMarkTM IFC Dynamic ArrayTM genotyping by quantitative PCR without any pre-amplification step (purple: WGA-FLDMqPCR). The markers are distributed along the X axis from the lowest to the highest retention frequencies obtained by the first method (the amplified panel with conventional agarose genotyping WGA-PCR in blue).
Rao et al. BMC Genomics 2012 13:513 doi:10.1186/1471-2164-13-513