Phylogenetic tree analysis based on alignment of the deduced amino acid sequences of pepper CaRGAs with known R genes. The tree was constructed by the neighbor-joining method using MEGA 5.0 software. The 51 CaRGA sequences and 2 resistance genes were grouped into seven subgroups: I–VII. I–IV are part of the non-TIR-NBS-LRR family, whereas V–VII belong to the TIR-NBS-LRR family. Bootstrap values (1000 replicates) are given below the branches. The known R genes with the NBS domain that were used were N (U15605), L6 (U27081), M (U73916), Prf (U65391), Gpa2 (AF195939) and RPM1 (X87851).
Wan et al. BMC Genomics 2012 13:502 doi:10.1186/1471-2164-13-502