Figure 7 .
Ligase activity of putative E3s was validated by in vivo assays. (A) HEK 293FT cells were transfected together with FLAG-E3s and HA-Ub. After MG-132 treatment, whole cell lysate was subjected to immunoprecipitation with anti-FLAG beads. Poly-ubiquitination was detected by Western blotting with anti-FLAG and anti-HA antibody. (B) Protein mixture was subjected to anti-FLAG beads after heating with 1% SDS.
Hou et al. BMC Genomics 2012 13:495 doi:10.1186/1471-2164-13-495