Figure 6 .
Ligase activity of putative E3s was validated by in vitro assays. Polyubiquitin chains were formed in the presence of UBE2D2 and Ube2k. Polyubiquitin chains were observed only in the presence of E1, E2, E3 and ubiquitin. GST protein was used as negative control (right lane6).
Hou et al. BMC Genomics 2012 13:495 doi:10.1186/1471-2164-13-495