Table 2

The detailed results of our testing on the Phalaenopsis ESTs data with different parameters
Parameters dT= 3,dN= 5,L= 21 dT= 4,dN= 5,L= 21 dT= 4,dN= 6,L= 21
Number of enumerated subsequences 191,087 191,087 191,087
Number of subsequences (afterFar_neighborand powerful subsequence examination) 173,106 188,889 117,203
Number of qualified siRNAs 100,890 106,188 6,443
Number of non-redundant qualified siRNAs 487 2,147 352
Average number of target genes of a qualified siRNA 1.0583 1.1163 1.0852
Number of qualified siRNAs (number of target gene > 1) 5,565 10,863 491
Average number of target genes of qualified siRNAs (number of target gene > 1) 2.0577 2.1370 2.1181
Number of selected siRNAs in first round analysis 142 94 162
Number of candidate-genes examined in first round analysis 229 229 227
Average examination frequency of each candidate-gene 1.153 1.170 1.110

From these result we can see that the number of qualified siRNA has important influence on the number of used siRNAs in first round analysis.

Chang et al.

Chang et al. BMC Genomics 2012 13:491   doi:10.1186/1471-2164-13-491

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