Using siRNA-G41 to simultaneously silence three target genes. The effects of silencing three putative transcription factors in plants that were transfected with a hairpin RNA-G41 are shown. The RNA levels of Pha106, Pha157 and PhaTF187 were analyzed in healthy (H) plants, in plants that were infiltrated with Agrobacterium (agro), in plants that were infiltrated with Agrobacterium that carried an empty pB7GWIW2 vector (agro-v) or in plants that were infiltrated with Agrobacterium that carried pB7GWIW2 to deliver hairpin RNAs designed from siRNA-G41 (hpRNA-G41). Phalaenopsis Ubiquitin 10 was used as an internal control.
Chang et al. BMC Genomics 2012 13:491 doi:10.1186/1471-2164-13-491