Flowchart of the design of the qualified siRNAs that were used in the first-round analysis of our strategy. The first three stages demonstrate the flow of the heuristic algorithm that we proposed. The qualified sequences can be derived by this heuristic algorithm, and the complement sequences of these qualified sequences are the qualified siRNAs. Based on the availability of the qualified siRNAs, we can then select the siRNAs for the first-round RNAi analysis from the qualified siRNAs in stage 4.
Chang et al. BMC Genomics 2012 13:491 doi:10.1186/1471-2164-13-491